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Techniques to Induce and Quantify Cellular Senescence
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Biologia
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JoVE Journal Biologia
Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

DOI:
10.3791/55533-v

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06:51 min

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May 01, 2017

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,
1Laboratory of Epidemiology and Population Science,National Institute on Aging, National Institutes of Health

Capítulos

  • 00:05Título
  • 00:43DNA Damage-induced Senescence
  • 01:40Cell Staining for Senescence-associated (SA) Beta-Galactosidase
  • 03:30Quantifying SA Secretory Phenotype (SASP) Protein Levels
  • 04:37Results: Representative SA-beta-Galactosidase Staining and SASP Expression Analyses
  • 05:42Conclusion

Summary

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Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence.

Tags

Cellular SenescenceSenescence InductionSenescence QuantificationGamma IrradiationSenescence-associated Beta-galactosidase AssayCell CultureMicroscopyImage Analysis

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