Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / High-resolution Imaging and Analysis of…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Biology
High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
Journal (Biology)
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants
Journal (Biology)
Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants
 

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Article DOI: 10.3791/55610-v • 10:23 min • April 20th, 2017
April 20th, 2017 •
1, 1, 1
1Department of Cell and Developmental Biology, University of Colorado School of Medicine

Capítulos

Resumo
Automatic Translation

Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.

Tags

High-resolution Imaging Analysis Individual Astral Microtubule Dynamics Budding Yeast Measure Microtubule Dynamics Yeast Cells Mutations In Tubules Microtubule Regulators Living Cell Technique Stabilized Yeast Cells Time-resolution Ten Minutes Several Hours Colby Fees Cassi Estrem Graduate Students GFP Labeled Tubulin Inoculate Yeast Cells Synthetic Complete Medium OD600 Flow Chamber Slides Paraffin Film Microscope Slide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter