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JoVE Journal Developmental Biology
Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
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Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Article DOI: 10.3791/55621-v • 09:04 min • April 22nd, 2017
April 22nd, 2017 •
1, 2, 1, 1,3
1Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, 2Department of Molecular Cardiology, Cleveland Clinic Foundation, 3Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

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We describe a technique for using murine embryonic stem cells for generating two or three dimensional embryoid bodies. We then explain how to induce neural differentiation of the embryoid body cells by retinoic acid, and how to analyze their state of differentiation by progenitor cell marker immunofluorescence and immunoblotting.

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Retinoic Acid-induced Neural Differentiation Mouse Embryonic Stem Cells Two-dimensional Embryoid Bodies Three-dimensional Embryoid Bodies Genes Protein Markers Neural Differentiation Mechanism Inhibition Of Neural Differentiation Stem Cells Into Neurons Therapeutic Purposes Optical Microscopy Dr. Junning Yang Embryoid Body Formation Trypsin-EDTA Iscove's Modified Dulbecco's Medium IMDM FBS Centrifuge Hemocytometer Retinoic Acid Concentration Petri Dish Plates
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