Ensaios de Retardamento de Gel de Agarose Protein-tRNA para Análise do<em> N</em<sup> 6</sup> -Treonilcarbamoyladenosina TcdA Function
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June 21, 2017
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Um protocolo é apresentado para a produção de tRNA (UUU) e a análise de tRNA (UUU) em complexo com a enzima TcdA por ensaios de retardamento de gel de agarose.
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Fernández, F. J., Gómez, S., Navas-Yuste, S., López-Estepa, M., Vega, M. C. Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function. J. Vis. Exp. (124), e55638, doi:10.3791/55638 (2017).
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