Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells <em>In Vivo</em>

Jolanta Jagodzinska; Emmanuelle Sarzi; M&#233;lanie Cavalier; Marie Seveno; Volker Baecker; Christian Hamel; Marie P&#233;quignot; Cecile Delettre

doi:10.3791/55865

Tomografia de coerência óptica: Imagens Mouse ganglionares da retina células In Vivo

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Neuroscience

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JoVE Journal Neuroscience

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Tomografia de coerência óptica: Imagens Mouse ganglionares da retina células In Vivo

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08:17 min

September 22, 2017

DOI: 10.3791/55865-v

Jolanta Jagodzinska*¹, Emmanuelle Sarzi*¹, Mélanie Cavalier¹, Marie Seveno¹, Volker Baecker², Christian Hamel^1,3,4, Marie Péquignot¹, Cecile Delettre¹

¹INSERM U1051, Institut of Neurosciences of Montpellier, ²CNRS UMS3426,BioCampus Montepellier, ³University of Montpellier, ⁴CHRU Montpellier, Centre of Reference for Genetic Sensory Diseases,CHU Gui de Chauliac Hospital

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Overview

This manuscript describes a protocol for the in vivo imaging of the mouse retina using high-resolution spectral domain optical coherence tomography (SD-OCT). The focus is on retinal ganglion cells (RGC) in the peripapillary region, employing various scanning and quantifying approaches.

Key Study Components

Area of Science

Neuroscience
Ophthalmology
Imaging Techniques

Background

Optical coherence tomography (OCT) is a non-invasive imaging technique.
It allows for the assessment of retinal thickness and structural changes.
Retinal ganglion cells are crucial for understanding optic neuropathy.
This method is safe and reputable for testing treatment effects.

Purpose of Study

To provide a protocol for imaging mouse retinal ganglion cells.
To assess retinal thickness accurately and quickly.
To explore the implications for optic neuropathy research.

Methods Used

Adjusting reference arm settings for the lens.
Utilizing high-resolution spectral domain optical coherence tomography.
Scanning the peripapillary region of the retina.
Quantifying retinal ganglion cell characteristics.

Main Results

Successful imaging of retinal ganglion cells in vivo.
Demonstrated the effectiveness of SD-OCT in assessing retinal thickness.
Provided insights into structural changes associated with optic neuropathy.
Validated the non-invasive nature of the technique.

Conclusions

The protocol offers a reliable method for retinal imaging.
It enhances understanding of retinal ganglion cell health.
This technique can aid in the evaluation of treatments for optic neuropathy.

Frequently Asked Questions

What is the main advantage of using SD-OCT?

The main advantage is that it is a non-invasive method for detecting structural changes in the retina.

How does OCT contribute to ophthalmology?

OCT helps in quickly obtaining an accurate assessment of retinal thickness and understanding optic neuropathy.

What are retinal ganglion cells?

Retinal ganglion cells are neurons in the retina that transmit visual information to the brain.

Is the imaging process safe?

Yes, the imaging process using SD-OCT is non-invasive and safe for the subjects.

What settings need to be adjusted before starting the imaging?

The reference arm settings appropriate for the lens must be adjusted before imaging.

Can this technique be used for other types of research?

While this study focuses on retinal ganglion cells, SD-OCT can be adapted for various ophthalmological studies.

Este manuscrito descreve um protocolo para a imagem no vivo da retina do rato com a tomografia de coerência óptica de domínio espectral de alta resolução (SD-OCT). Centra-se em células ganglionares da retina (RGC) na região de peripapillary, com vários digitalização e quantificar as abordagens descritas.

O objetivo geral deste experimento é usar tomografia de coerência óptica ou OCT para obter rapidamente uma avaliação precisa da espessura da retina. A OCT pode ajudar a responder a questões-chave no campo da oftalmologia, como neuropatia óptica. A principal vantagem desta técnica é que é um método não invasivo para detectar alterações estruturais nas células ganglionares da retina de camundongos.

Esta técnica é realmente interessante porque é um método não invasivo, seguro e respeitável para testar o efeito do tratamento na neuropatia óptica. Para começar, primeiro ajuste as configurações do braço de referência apropriadas para a lente. Em seguida, ligue a fonte de alimentação e o computador.

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