Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Determination of the Optimal Chromosomal…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Journal (Genetics)
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
Journal (Genetics)
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
 

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Article DOI: 10.3791/55946-v • 11:12 min • September 11th, 2017
September 11th, 2017 •
1, 1, 2, 1
1Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP), University of Copenhagen, 2Department of Microbiology and Infection Control, Statens Serum Institut

Capítulos

Resumo
Automatic Translation

Here, the power of a transposon-mediated random insertion of a non-coding DNA element was used to resolve its optimal chromosomal position.

Tags

Optimal Chromosomal Location DNA Element Escherichia Coli Transposon-mediated Approach Competition Experiments Whole Genome Sequencing DNA Replication Genetic Element Fittest Clone Growth Advantage E.coli DARS2 Region Electrocompetant Cells PNKBOR DARS2 Electroporation Chamber Bacterial Suspension LB Broth
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter