Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Induction of Paralysis and Visual System Injury…
JoVE Journal
Immunology and Infection

This Conteúdo is Open Access.

JoVE Journal Immunology and Infection
Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Journal (Immunology and Infection)
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Dual-Reporter IgG and IgM Serological Assay for Simultaneous Antibody Detection of Borrelia (Video) | JoVE
Journal (Immunology and Infection)
Dual-Reporter IgG and IgM Serological Assay for Simultaneous Antibody Detection of Borrelia (Video) | JoVE
 

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Article DOI: 10.3791/56185-v • 09:29 min • August 21st, 2017
August 21st, 2017 •
*1,2, *1, 1,2, 3, 3, 1, 4, 1,2
1Department of Neurology, University of California, 2Program in Immunology, University of California, 3Department of Neurology and Neurological Sciences, Stanford University, 4Department of Pathology, Stanford University
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Here, we present a protocol to induce paralysis and opticospinal inflammation by transfer of aquaporin-4 (AQP4)-specific T cells from AQP4-/- mice into WT mice. In addition, we demonstrate how to use serial optical coherence tomography to monitor visual system dysfunction.

Tags

Induction Paralysis Visual System Injury Mice T Cells Neuromyelitis Optica Aquaporin-4 CNS Autoimmune Disease Adoptive Transfer Aquaporin 4-specific T Cells Dynamic Visual System Optical Coherence Tomography Retinal Injury Noninvasive Monitoring AQP4-specific T Cells Inflammatory Visual Disorders Procedure Nylon Stopcock Luer Lock Connections Glass Luer Lock Syringe Plunger Peptide Complete Freund's Adjuvant Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter