Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Quantification of the Abundance and Charging…
JoVE Journal
Biochemistry

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Biochemistry
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
Journal (Biochemistry)
Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Journal (Biochemistry)
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
 

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Article DOI: 10.3791/56212-v • 10:34 min • August 22nd, 2017
August 22nd, 2017 •
1, 1, 1
1Department of Biology, University of Copenhagen

Capítulos

Resumo
Automatic Translation

Here we present a method for directly measuring transfer RNA charging levels from purified Escherichia coli RNA as well as a way to compare relative levels of transfer RNA, or any other short RNA, across different samples based on the addition of spike-in cells expressing a reference gene.

Tags

Quantification Abundance Charging Levels Transfer RNAs Escherichia Coli Experiment Cell Biology Growth States RNA Levels Composition Spike-in Culture TRNA Cell C IPTG OD436 Harvesting TCA Enzymatic Activities
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter