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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Article DOI: 10.3791/56226-v • 09:45 min • August 10th, 2017
August 10th, 2017 •
1, 2, 2, 2
1Department of Molecular Biology, Rowan School of Osteopathic Medicine, 2Department of Biological Sciences, Rowan University

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Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.

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MRNA Levels Yeast S. Cerevisiae Hypoxic Response Experiment Cell Signaling Gene Expression Changes Kinetics MRNA Measurement Microbial Species Liquid Culture Visual Demonstration Flasks Tubing Connections Collection Of Cells Nitrogen Tank Incubator Vacuum Filtering System YPD Media Autoclaving Layout Planning
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