Establishment of Larval Zebrafish as an Animal Model to Investigate <em>Trypanosoma cruzi</em> Motility <em>In Vivo</em>

Veronica Akle; Nathalie Agudelo-Due&#241;as; Maria A. Molina-Rodriguez; Laurel Brianne Kartchner; Annette Marie Ruth; John M. Gonz&aacute;lez; Manu Forero-Shelton

doi:10.3791/56238

Journal

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Biologia do Desenvolvimento

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Estabelecimento de Zebrafish Larval como um modelo Animal para investigar Trypanosoma cruzi motilidade In Vivo

Establishment of Larval Zebrafish as an Animal Model to Investigate <em>Trypanosoma cruzi</em> Motility <em>In Vivo</em>

JoVE Journal
Biologia do Desenvolvimento
Author Produced

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JoVE Journal Biologia do Desenvolvimento

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

Estabelecimento de Zebrafish Larval como um modelo Animal para investigar Trypanosoma cruzi motilidade In Vivo

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10,552 Views

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13:21 min

•

September 30, 2017

DOI:

10.3791/56238-v

DOI:

10.3791/56238-v

•

13:21 min

September 30, 2017

•

3 Views

Veronica Akle¹, Nathalie Agudelo-Dueñas*^1,2, Maria A. Molina-Rodriguez*¹, Laurel Brianne Kartchner^1,3,4,6, Annette Marie Ruth^1,3,5,6, John M. González³, Manu Forero-Shelton²

¹Laboratory of Neurosciences and Circadian Rhythms, School of Medicine,Universidad de los Andes, ²Biophysics Group, Department of Physics,Universidad de los Andes, ³Laboratory of Basic Medical Sciences, School of Medicine,Universidad de los Andes, ⁴Department of Microbiology and Immunology,University of North Carolina, ⁵Notre Dame Initiative for Global Development,University of Notre Dame, ⁶USAID Research and Innovation Fellowship program

Transcrição

Summary

Neste protocolo, fluorescente etiquetadas T. cruzi foram injetados em larvas de peixe-zebra transparente, e motilidade do parasita foi observada na vivo usando microscopia de fluorescência folha de luz.

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