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Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

DOI:
10.3791/56352-v

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10:06 min

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August 03, 2017

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, , ,
1Graduate School of Medical Sciences,Kanazawa University

Capítulos

  • 00:05Título
  • 00:58Stage 16 Embryo Collection
  • 02:39Embryonic Preparation
  • 04:50Hemocyte and TUNEL Staining
  • 07:41Results: Representative Hemocyte and Apoptotic Cell Staining
  • 09:03Conclusion

Summary

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We herein describe a phagocytosis assay using the dispersed embryonic cells of Drosophila. It enables us to easily and precisely quantify in vivo phagocytosis levels, and to identify new molecules required for the phagocytosis of apoptotic cells.

Tags

PhagocytosisApoptotic CellsDrosophila EmbryosImmune ResponseBiologia do DesenvolvimentoCell EngulfmentInflammatory DisordersAutoimmune DiseaseCell IsolationCollagenaseCell SuspensionCell HomogenizationCell Trituration

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