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Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Article DOI: 10.3791/56514-v • 10:33 min • October 25th, 2017
October 25th, 2017 •
1,2, 2, 2, 1,2
1Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, 2Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne

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We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.

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Isolation Salmonella Typhimurium Phagosomes Macrophages Immunology Antigen Processing Pathogenic Microorganisms Technique High Quality Simple Steps Specialized Equipment Mechanism Intracellular Pathogens Culture Brain Heart Infusion Broth Incubator Bacterial Suspension Conical Flask Centrifuge
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