Improved 3D Hydrogel Cultures of Primary Glial Cells for <em>In Vitro</em> Modelling of Neuroinflammation

Kyle M. Koss; Matthew A. Churchward; Andrea F. Jeffery; Vivian K. Mushahwar; Anastasia L. Elias; Kathryn G. Todd

doi:10.3791/56615

Melhorado o hidrogel 3D culturas de células gliais primárias para In Vitro modelagem de ...

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JoVE Journal Bioengineering

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Melhorado o hidrogel 3D culturas de células gliais primárias para In Vitro modelagem de Neuroinflammation

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09:19 min

December 8, 2017

DOI: 10.3791/56615-v

Kyle M. Koss^1,2, Matthew A. Churchward^1,2, Andrea F. Jeffery^1,3, Vivian K. Mushahwar^1,2,4, Anastasia L. Elias³, Kathryn G. Todd^1,2,5

¹Department of Psychiatry,University of Alberta, ²Alberta Innovates-Health Solutions Interdisciplinary Team in Smart Neural Prostheses (Project SMART),University of Alberta, ³Department of Chemical and Materials Engineering,University of Alberta, ⁴Division of Physical Medicine and Rehabilitation,University of Alberta, ⁵Centre for Neuroscience,University of Alberta

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Neste documento, apresentamos um protocolo para a cultura 3D do rato as células da glia derivado do cérebro, incluindo astrócitos, oligodendrócitos e micróglia. Vamos demonstrar a cultura de células primárias, síntese de hidrogel de ácido hialurônico methacrylated (HAMA), encapsulamento HAMAphoto-polimerização e celular e amostra de processamento de imagem microscópica confocal e varredura de elétrons.

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Bioengenharia edição 130 cultura de células 3D hidrogel glia primário astrocyte microglia oligodendrocyte neuroinflammation ácido hialurônico HAMA