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JoVE Journal Genetics
Övergående uttryck av främmande gener i insekt celler (sf9) för Protein funktionell analys
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Övergående uttryck av främmande gener i insekt celler (sf9) för Protein funktionell analys

Article DOI: 10.3791/56693-v • 11:12 min • February 22nd, 2018
February 22nd, 2018 •
1, 2, 2, 3, 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

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Det här protokollet beskriver en värme chock-inducerad protein uttryck systemet (pDHsp/V5-hans/sf9 cellsystem), som kan användas för att uttrycka främmande proteiner eller utvärdera anti-apoptotiska aktiviteten av potentiella främmande proteiner och deras trunkerade amino syror i insekt celler.

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Genetik fråga 132 övergående uttryck protein funktionell analys sf9 cellinje Lymantria xylina flera nukleopolyedervirus Ly-inhinbitor av apoptos-3 Drosophila värme chock 70 promotorn pDHsp/V5-hans Drosophilia Reaper actinomycin-D
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