Journal
/
Biologia
/
Primary Cell Cultures from the Mouse Retinal Pigment Epithelium
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

Primary Cell Cultures from the Mouse Retinal Pigment Epithelium

DOI:
10.3791/56997-v

•

09:35 min

•

March 16, 2018

•

, , , ,
1Department of Ophthalmology,University of Pittsburgh School of Medicine, 2Wilmer Eye Institute,The Johns Hopkins University School of Medicine

Capítulos

  • 00:04Título
  • 00:50Isolating and Preparing Eyeballs for Dissection
  • 03:18Isolating the RPE-choroid-sclera Complex from Eyeballs
  • 05:07Collecting and Culturing Primary RPE Cells from RPE-choroid-sclera Complexes
  • 07:22Results: Characterize Mouse Primary RPE Cultures
  • 08:59Conclusion

Summary

Tadução automática

Tadução automática

The retinal pigment epithelium (RPE) is a multi-functional epithelium of the eye. Here we present a protocol to establish primary cell cultures derived from the murine RPE.

Tags

Primary Cell CultureMouse Retinal Pigment EpitheliumRPE IsolationRPE Cell CultureEuthanasiaEye DissectionDispase IILaminar Flow CabinetGrowth MediumMicroscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

Primary Cell Cultures from Drosophila Gastrula Embryos

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Isolation of Primary Mouse Retinal Pigmented Epithelium Cells

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies

Read Article