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JoVE Journal Immunology and Infection
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Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Article DOI: 10.3791/57514-v • 07:37 min • May 22nd, 2018
May 22nd, 2018 •
*1, *1, 1,2, 1, 1, 2, 1
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 2Tianjin Agricultural University
* These authors contributed equally

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Here, we present a protocol to describe extracellular vesicles (EVs) isolation in in vitro cultured medium from adult Schistosoma japonicum.

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Isolation Characterization Extracellular Vesicles Adult Schistosoma Japonicum In Vitro Culture Technique Stress Minimization Juntao Liu Technician Parasites Beaker Washes PBS Gravity Sedimentation Worm Pairs RPMI Medium Antibiotics Culture Temperature Carbon Dioxide Centrifuge Tubes Eggs Debris Supernatant Syringe Filter
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