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JoVE Journal Developmental Biology
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Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Article DOI: 10.3791/57599-v • 09:33 min • June 2nd, 2018
June 2nd, 2018 •
1, 2,3,4, 2,3,4, 1,2,3,4,5
1Program in Developmental Biology, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children's Hospital, and Houston Methodist Hospital, Baylor College of Medicine, 3Molecular and Cellular Biology Department, Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, 5McNair Medical Institute, Baylor College of Medicine

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Here, we present a protocol to generate insulin expressing 3D murine pancreatoids from free-floating e10.5 dissociated pancreatic progenitors and the associated mesenchyme.

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Scaffold-free Three-dimensional Insulin Expressing Pancreatoids Mouse Pancreatic Progenitors In Vitro Endocrine Cells Architecturally Complex Environment Cellularly Complex Environment Mesenchyme Dissection E10.5 Ice Bucket Phosphate Buffered Saline Fine-tip Forceps Dissection Scissors 70% Ethanol Borosilicate Capillary Tubes Lighter Filtered 1000 Microliter Pipet Tips Dispase Sterilized Water Trypsin E10.5 Timed Pregnant Mice IACUC Guidelines
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