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Een stroom Cytometry gebaseerde Assay voor het meten van de Mitochondriale membraanpotentiaal in cardiale Myocytes na Hypoxia/heroxygenatie
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Een stroom Cytometry gebaseerde Assay voor het meten van de Mitochondriale membraanpotentiaal in cardiale Myocytes na Hypoxia/heroxygenatie

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Hier presenteren we een protocol dat gebruikmaakt van JC-1 kleurstof te beoordelen van de Mitochondriale membraanpotentiaal van cellen na wordt blootgesteld aan hypoxie/heroxygenatie met of zonder een beschermende agent.

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Gedrag kwestie 137 mitochondriale permeabiliteit overgang porie mitochondriaal membraanpotentiaal JC-1 assay reperfusie letsel cardiale myocytes stroom cytometry
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