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JoVE Journal Immunology and Infection
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Article DOI: 10.3791/57804-v • 10:28 min • July 17th, 2018
July 17th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1
1Department of Physiological Sciences, Eastern Virginia Medical School

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The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.

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Isolation Expansion Adipogenic Induction CD34+CD31+ Endothelial Cells Human Omental Adipose Tissue Subcutaneous Adipose Tissue Obesity Field Adipose Tissue Remodeling Key Factors Mechanisms Robust Adipogenic Response Technique Small Amounts Of Tissue CD34+CD31+ Endothelial Cell Isolation Human Adipose Vasculature Adipogenic Stimulation Cell Isolation Steps Fresh Human Adipose Tissue Omental And Subcutaneous Adipose Tissue Collection Bariatric Surgery Hank's Buffered Salt Solution Penicillin Streptomycin Fibrotic And Cauterized Sections Removal Collagenase Digestion
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