Enriquecimento de Fosfopeptida acoplado com espectrometria de massa livre de rótulo quantitativa para investigar o Phosphoproteome no câncer de próstata

14K visualizações

Citado por 15

12:23 min.

2 de agosto de 2018

10.3791/57996-v

2 de agosto de 2018

14K visualizações

Larry C. Cheng^*¹^,² , Zhen Li^*³ , Thomas G. Graeber⁴ , Nicholas A. Graham⁵ , Justin M. Drake¹^,²^,³^,⁶^,⁷

¹Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, ²Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, ³Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, ⁴Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, ⁵Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, ⁶Pharmacology, Rutgers Robert Wood Johnson Medical School, ⁷Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey

^* These authors contributed equally

Este protocolo descreve um procedimento para extrair e enriquecer o phosphopeptides de linhas de células de câncer de próstata ou tecidos para uma análise da phosphoproteome através de massa baseado em espectrometria proteômica.

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