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Journal / Genetics / CRISPR Guide RNA Cloning for Mammalian Systems
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JoVE Journal Genetics
CRISPR Guide RNA Cloning for Mammalian Systems
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CRISPR Guide RNA Cloning for Mammalian Systems

Article DOI: 10.3791/57998-v • 06:48 min • October 2nd, 2018
October 2nd, 2018 •
*1,2, *1,2,3, 1,2, 1,2, 1, 1, 1,4,5,6,7, 1,2
1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute
* These authors contributed equally

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Here, a simple, efficient, and cost-effective method of sgRNA cloning is outlined.

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CRISPR Guide RNA Cloning Mammalian Systems CRISPR-facilitated Experiments Single-guide RNA Expression Plasmids Primers PCR Strip Cap Tubes Guide RNA Oligonucleotide Mixtures PSB700 Guide Expression Vector BSNB1 Ligation Digestion Products Low Melting Agarose Gel DNA Extraction Elute
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