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Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
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Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology

Article DOI: 10.3791/58031-v • 08:17 min • July 3rd, 2018
July 3rd, 2018 •
1, 1, 2, 1
1School of Pharmacy, University of Wyoming, 2Department of Zoology and Physiology, University of Wyoming

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Resumo
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Here we present a protocol to visualize fine structures of peripheral nerves by obtaining and staining 1-2 µm sections with toluidine blue

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Toluidine Blue Staining Resin-embedded Sections Peripheral Nerve Morphology Morphometry Axon Count Myelination State Fibrotic Tissue High-resolution Visualization Anesthetized Rat Dissection Mat Anesthesia Maintenance Isoflurane Pedal Withdrawal Reflex Palpebral Reflex Hind Limb Hair Shaving Sterilization With Ethanol Femur Palpation Incision Location Biceps Femoris Muscle Gluteus Maximus Muscle Microdissection Scissors Sciatic Nerve Exposure
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