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JoVE Journal Bioengineering
Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography
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Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

Article DOI: 10.3791/58040-v • 11:12 min • October 3rd, 2018
October 3rd, 2018 •
1, 2, 1, 2, 2, 3
1Department of Cardiology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, 2Medical Research Center, Guangdong Academy of Medical Sciences, Guangdong General Hospital, 3Institute of Geriatric medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital

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A step by step protocol for nondestructive and long-period monitoring the process of vascular remodeling and scaffold degradation in real-time culture of biodegradable polymeric scaffold-based tissue-engineered blood vessels with pulsatile stimulation using optical coherence tomography is described here.

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Nondestructive Monitoring Degradable Scaffold Tissue-engineered Blood Vessel Optical Coherence Tomography OCT Vascular Growth Remodeling Structural Features Imaging Strategy Long-term Culture PGA Scaffold Sodium Hydroxide Tissue-culture-grade Water Bioreactor Y-junction
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