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JoVE Journal Genetics
Eneste dråpe digitale polymerasekjedereaksjons omfattende og samtidige deteksjon av mutasjoner i Hotspot regioner
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Eneste dråpe digitale polymerasekjedereaksjons omfattende og samtidige deteksjon av mutasjoner i Hotspot regioner

Article DOI: 10.3791/58051-v • 08:23 min • September 25th, 2018
September 25th, 2018 •
1,2, 1, 1, 1,3,4, 1,3,5, 1,6, 1
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, 2CNRS UMR144, Institut Curie, PSL Research University, 3Department of Medical Oncology, Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830, Institut Curie, PSL Research University

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Her presenterer vi en protokoll for å nøyaktig kvantifisere flere genetiske endringer av målet regionen i en enkelt reaksjon drop-off ddPCR og en unik par hydrolyse sonder.

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Genetikk problemet 139 Drop-off ddPCR Hotspot mutasjoner kreft sirkulerende svulst DNA diagnostikk
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