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Immunology and Infection

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Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Article DOI: 10.3791/58061-v • 07:21 min • July 29th, 2018
July 29th, 2018 •
1,2, 1,2
1Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Research Division of Immunology, Cedars-Sinai Medical Center

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We demonstrate how to identify and isolate 6 subsets of myeloid progenitors from murine bone marrow using a combination of magnetic and fluorescence sorting (MACS and FACS). This protocol can be used for in vitro culture assays (methylcellulose or liquid cultures), in vivo adoptive transfer experiments, and RNA/protein analyses.

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Identification Isolation Oligopotent Lineage-committed Myeloid Progenitors Mouse Bone Marrow Hematologists Immunologists Myeloid Cells Neutrophils Monocytes Dendritic Cells Technique Precise Identification Subsets Strategies Bone Marrow Cells Magnetic-activated Cell Sorting MACS Centrifugation Resuspend MACS Staining Buffer Biotin Antibody Cocktail Incubation Four Degrees Celsius Anti-biotin Microbeads Wash Cells Automated Magnetic Separator Negative Selection
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