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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Article DOI: 10.3791/58081-v • 09:07 min • October 30th, 2018
October 30th, 2018 •
1,2, 1,2,3, 1,2, 1
1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, 2Faculty of Science, Charles University, 3Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

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In this article, we provide a detailed protocol for the expression of fluorescent fusion proteins in murine bone marrow derived dendritic cells and macrophages. The method is based on the transduction of bone marrow progenitors with retroviral constructs followed by differentiation into macrophages and dendritic cells in vitro.

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Fluorescent Fusion Proteins Murine Bone Marrow-derived Dendritic Cells Macrophages Sub-cellular Localization Protein Dynamics Technique Advantages Simple Method Inexpensive Method Efficiency Of Expression Stability Of Expression Retrovirus Production Platinum Eco-packaging Cells DMEM Culture Medium Fetal Bovine Serum (FBS) Retroviral Construct PCL Eco-packaging Vector PEI Transfection Reagent
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