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TChIP-Seq: Cell-Type-Specific Epigenome Profiling
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07:28 min
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January 23, 2019
DOI:
1RNA Biology Laboratory,RIKEN, 2RNA Systems Biochemistry Laboratory,RIKEN Cluster for Pioneering Research, 3Laboratory for Phyloinformatics,RIKEN Center for Biosystems Dynamics Research, 4RNA Biology Laboratory, Faculty of Pharmaceutical Sciences,Hokkaido University, 5Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences,University of Tokyo
Summary
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We describe a step-by-step protocol for tandem chromatin immunoprecipitation sequencing (tChIP-Seq) that enables the analysis of cell-type-specific genome-wide histone modification.
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Mito, M., Kadota, M., Nakagawa, S., Iwasaki, S. TChIP-Seq: Cell-Type-Specific Epigenome Profiling. J. Vis. Exp. (143), e58298, doi:10.3791/58298 (2019).
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