Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Dandan Li; H&#243;ngyi Zh&#224;o; Wei Wei; Nan Liu; Yonghua Dr. Huang

doi:10.3791/58423

Subaguda Microhemorrhages Cerebral induzida pela injeção de lipopolissacarídeo em ratos

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JoVE Journal Neuroscience

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Subaguda Microhemorrhages Cerebral induzida pela injeção de lipopolissacarídeo em ratos

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06:39 min

October 17, 2018

DOI: 10.3791/58423-v

Dandan Li*^1,2, Hóngyi Zhào*^2,3, Wei Wei², Nan Liu², Yonghua Dr. Huang²

¹Department of Neurology,Second Hospital of Shanxi Medical University, ²Department of Neurology,PLA Army General Hospital, ³Department of Neurology,NO 261 Hospital of PLA

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Overview

This study presents a protocol for inducing and detecting cerebral micro-hemorrhages (CMHs) in Sprague-Dawley rats through LPS injection. The work contributes to understanding the pathogenesis of CMHs and may inform future research into various brain disorders.

Key Study Components

Area of Science

Neuroscience
Pathophysiology
Animal Models

Background

Cerebral micro-hemorrhages are associated with various neurological disorders.
Inflammation plays a critical role in the development of CMHs.
LPS is commonly used to induce inflammation in experimental models.
The technique may extend to studying other brain disorders like Parkinson's and Alzheimer's disease.

Purpose of Study

To develop a reliable method for inducing CMHs in a laboratory setting.
To explore the inflammatory processes involved in CMH pathogenesis.
To establish a basis for future research on brain disorders.

Methods Used

LPS is administered via intraperitoneal injection at a dose of 1 mg/kg.
The animal model used is 10-week-old male Sprague-Dawley rats.
Cardiac puncture and Evans blue dye are used for assessing blood-brain barrier integrity.
The protocol includes multiple injections and specific tissue preparation steps for imaging.
Various staining techniques are applied for analyzing brain tissue.

Main Results

The LPS injection reliably induces CMHs, observable through various imaging techniques.
Methods allow the visualization of red blood cells outside blood vessels via H&E staining, indicating CMH presence.
Evans blue staining indicates compromised blood-brain barrier function and highlights CMH distribution.

Conclusions

This study demonstrates a straightforward method for investigating CMHs and their underlying mechanisms.
Insights gained could enhance understanding of CMHs and related neurological disorders.
The protocol also enables researchers to examine inflammation's role in cerebral injury.

Frequently Asked Questions

What are the advantages of using LPS injections in this model?

LPS injections induce stable inflammation, making them an effective method for studying CMHs and related processes in the brain.

How is the biological model implemented for studying CMHs?

The model involves administering LPS to Sprague-Dawley rats, followed by specific imaging and staining techniques to assess CMH presence.

What types of data are obtained from this method?

Key outcomes include visualization of CMHs, assessment of blood-brain barrier integrity, and molecular readouts from tissue staining.

In what ways can this method be adapted for other studies?

While focused on CMHs, the technique can also be applied to research on other brain disorders like depression and Alzheimer’s disease.

Are there any limitations or considerations when using this protocol?

Mortality rates can vary based on the age and health of the rats; thus, maintaining a clean environment is crucial to minimize risk.

Apresentamos um protocolo para induzir e detectar CMHs causadas pela injeção de LPS em ratos Sprague-Dawley, que pode ser utilizaram no futuro pesquisa investigações na patogênese do CMHs.

Este método pode ajudar a responder a perguntas-chave no campo da micromorragem cerebral, como a etiologia, distribuição e detecção de micromorragem cerebral e assim por diante. A principal vantagem desta técnica é que as injeções de LPS induzem a inflamação. Além disso, a injeção de LPS é bastante simples, econômica e econômica.

Embora este método possa fornecer uma visão da indução da hemorragia cerebral, ele também pode ser aplicado a outros distúrbios cerebrais, como depressão, esquizofrenia, doença de Parkinson, doença de Alzheimer e doença de prída. Administre LPS a uma dose de um miligrama por quilograma de peso corporal para ratos sprague dawley machos de 10 semanas de idade por injeção intraperitoneal, em seguida, retornar cada rato para a gaiola doméstica. Repita a injeção de LPS após seis horas e 16 horas.

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