Isolamento de CD4+ T-células e análise de circulação folicular-T Helper (cTfh) célula subconjuntos de citometria de fluxo de 6 cores usando o sangue periférico

Overview

This article presents a protocol for isolating and characterizing CD4 + T-cell subsets from human peripheral blood. It emphasizes the use of flow cytometry to analyze the proportions of T-follicular helper cell subsets in different health states.

Key Study Components

Research Area

• Immunology
• T-cell biology

Background

• CD4 + T-cells play a crucial role in the adaptive immune response.
• T-follicular helper cells are vital for B-cell responses and antibody production.

Methods Used

• Flow cytometry for cell subset analysis
• Human peripheral blood as the biological system
• Batch processing technique for sample analysis

Main Results

• Identification of differences in T-follicular helper cell subsets between healthy and diseased individuals.
• Proven correlation of T-follicular helper cell subsets with clinical disease markers.
• Verification of method efficacy and practicality in clinical settings.

Conclusions

• This study demonstrates a reliable method for evaluating T-follicular helper subsets in various health statuses.
• The findings are significant for understanding disease progression and potential biomarkers.

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