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JoVE Journal Developmental Biology
Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
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Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Article DOI: 10.3791/58524-v • 07:25 min • March 20th, 2019
March 20th, 2019 •
1, 1
1Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Centro de Investigación y Tecnología Agroalimentaria de Aragón

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We present a methodology to quantify the starch content in the ovary primordia in sweet cherry (Prunus avium L.) during winter dormancy by using an image analysis system combined with histochemical techniques.

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Histochemical Staining Image Analysis Quantify Starch Ovary Primordia Sweet Cherry Winter Dormancy Woody Plant Dormancy Flower Buds Cold Accumulation Starch Quantification Small Samples Flower Primordium Physiological Activity Tree Species Apricot Plum Reproductive Phase Pollination To Fruiting Onset Collect Shoots Fixative Solution 75% Ethanol Growth Chamber Evaluate Bud Growth External Bud Scales
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