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Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

Article DOI: 10.3791/58845-v • 09:57 min • March 14th, 2019
March 14th, 2019 •
*1,2,3, *1,2, 4, 2, 1, 1,2, 1,2,3
1The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, 2CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 3CAS Key Laboratory of Infection and Immunity (CASKLII), Institute of Biophysics, Chinese Academy of Sciences, 4Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
* These authors contributed equally

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This protocol describes how to establish viral infection in vivo in Drosophila melanogaster using the nano-injection method and basic techniques to analyze virus-host interaction.

Tags

Viral Infection Host-virus Interaction Drosophila Melanogaster Nanoinjection Method Infection Dose Microbial Pathogens S2 Star Cells Schneider's Drosophila Medium Drosophila C Virus DCV Cell Culture Dishes Virus Collection Supernatant Lysis Of Host Cells Working Concentrations Of Virus
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