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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example
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Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

Article DOI: 10.3791/58965-v • 14:23 min • February 16th, 2019
February 16th, 2019 •
1, 1
1Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

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This article provides a method to isolate pure embryonic tissues from quail and chicken embryos that can be combined to form ex vivo chimeric organs.

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Isolation Embryonic Tissues Quail Chicken Chimeric Organs Thymus Example Experimental Approach Gene Expression Studies Functional Assays Microsurgery Enzymatic Digestion Three-dimensional Preserved Tissues Topographical Gene Expression Patterns Analysis Transcriptome Analysis Approaches Genetic Markers High Throughput Omic Analysis In Vitro Organotypic Assay Tissue Interactions Nuclear Features Molecular Markers Developmental Processes
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