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An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

Article DOI: 10.3791/58966-v • 09:47 min • February 1st, 2019
February 1st, 2019 •
1, 1
1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University

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Here, we present a protocol for a novel gap junction intercellular communication assay designed for the high-throughput screening of gap junction-modulating chemicals for drug discovery and toxicological assessment.

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Iodide-YFP-GJIC Assay High-throughput Screening Gap Junction Modulators Gap Junction Activities Simple Assay Robust Assay Repeatable Assay Inexpensive Assay Acceptor Cells Donor Cells Balanced Salt Solutions Lucifer Yellow Calcein AM Single Well Variability LN215 Cells Supplemented DMEM Trypsin EDTA Cell Density Culture Medium 24-well Culture Plate Lentivirus Polybrene
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