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Journal / Bioengineering / 三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定…
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JoVE Journal Bioengineering
三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定
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三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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本文提出了一种用荧光基质金属蛋白酶 (mmp) 可降解肽对聚 (乙二醇) (peg) 水凝胶进行包封和培养的方案。细胞 mmp 和代谢活性直接从水凝胶培养物测量使用标准的微板读取器。

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生物工程 第143期 水凝胶 三维细胞包封 基质金属蛋白酶 代谢活性 黑色素瘤 荧光
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