Breath Collection from Children for Disease Biomarker Discovery

Amalia  Z. Berna; Brian DeBosch; Janis Stoll; Audrey  R. Odom John

doi:10.3791/59217

Coleção de respiração das crianças para a descoberta de biomarcador de doença

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Chemistry

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JoVE Journal Chemistry

Breath Collection from Children for Disease Biomarker Discovery

Coleção de respiração das crianças para a descoberta de biomarcador de doença

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06:09 min

February 14, 2019

DOI: 10.3791/59217-v

Amalia Z. Berna¹, Brian DeBosch^1,2, Janis Stoll³, Audrey R. Odom John^1,4

¹Department of Pediatrics,Washington University School of Medicine, ²Cell Biology & Physiology,Washington University School of Medicine, ³Division of Gastroenterology, Hepatology and Nutrition,Washington University School of Medicine, ⁴Department of Molecular Microbiology,Washington University School of Medicine

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Overview

This protocol outlines a noninvasive method for collecting breath samples from children, which can be analyzed for biomarkers of various diseases. The technique is designed to be simple and reproducible, facilitating comparisons across studies.

Key Study Components

Area of Science

Neuroscience
Biology
Pediatrics

Background

Breath analysis can provide insights into pediatric medical conditions.
The method is noninvasive, making it suitable for children.
Standardized techniques enhance the reliability of results.
Visual instructions aid in the setup of complex equipment.

Purpose of Study

To develop a method for noninvasive breath sample collection in children.
To facilitate the diagnosis of various diseases.
To enable comparisons of results across different studies.

Methods Used

Collection of mixed air samples using sorbent tubes.
Gas chromatography-mass spectrometry for analysis.
Assembly of breath sample equipment with specific components.
Use of visual aids for equipment setup.

Main Results

Successful collection of breath samples from children.
Identification of biomarkers related to infections and metabolic disorders.
Demonstration of the method's reproducibility and simplicity.
Potential applications in point-of-care testing.

Conclusions

The breath collection method is effective for pediatric use.
It opens avenues for noninvasive diagnostics in various diseases.
Standardization can improve research comparability.

Frequently Asked Questions

What are the advantages of this breath collection method?

It is noninvasive, simple, and reproducible, making it suitable for children.

What diseases can be diagnosed using this method?

It can identify biomarkers for infections, metabolic disorders, and cancer.

How is the breath sample collected?

Samples are collected using sorbent tubes attached to a breath sampler.

Is visual instruction necessary for this method?

Yes, visual aids help in setting up the complex equipment.

Can this method be used for clinical testing?

Yes, it has potential applications for point-of-care testing.

How does this method compare to traditional sampling techniques?

It is less invasive and allows for easier sample collection from children.

Este protocolo descreve um método simples para a aquisição das amostras de respiração dos filhos. Brevemente, amostras de ar misto pré-concentram-se em tubos de adsorvente antes da análise por cromatografia gasosa / espectrometria de massa. Biomarcadores de respiração de doenças infecciosas e não-infecciosa podem ser identificados usando este método de coleta de respiração.

Um método padronizado de coleta de respiração em crianças poderia ser usado para responder a perguntas-chave sobre o estado das condições médicas pediátricas de forma não invasiva e comparar resultados entre os estudos. A principal vantagem dessa técnica é que permite a coleta de amostras de respiração de crianças de forma simples, não invasiva e reprodutível. As implicações dessas técnicas se estendem ao diagnóstico de uma ampla gama de estados da doença, incluindo infecções, distúrbios metabólicos e câncer e para o ponto de atenção ao uso clínico.

A instrução visual é muito útil porque há tantas peças em nossos equipamentos de coleta de respiração e porque a instalação do equipamento pode ser complicada. Para montar o equipamento de amostra de respiração, conecte um bocal de papelão ao amostrador de respiração e conecte um curto comprimento de tubo de grande diâmetro à outra extremidade do amostrador. Conecte o conector da respiração à válvula do saco através da tubulação.

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