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Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
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Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Article DOI: 10.3791/59335-v • 07:40 min • August 9th, 2019
August 9th, 2019 •
*1, *2, 2, 1
1Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, 2Center for Gastrointestinal Biology, Federal University of Minas Gerais
* These authors contributed equally

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This protocol describes a technique to image different cell populations in draining lymph nodes without alterations in the organ structure.

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Lymph Node Structure Cellular Localization Ex-vivo Confocal Microscopy Fluorescent Labeled Antibodies Secondary Lymphoid Organs Protocol Visualization Execution Mouse Handling Tissue Preservation Cell Viability Fluorescent Staining Methods Technician Dilute Antibodies Fluorophores PBS Inguinal Lymph Node Imaging Popliteal Lymph Node Imaging Syringe Needle Subcutaneously Inject Experimental Mouse Experimental Animal
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