Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Liang Wen; Wendong You; Yadong Wang; Yuanrun Zhu; Hao Wang; Xiaofeng Yang

doi:10.3791/59410

Investigando alterações na microbiota de caecum após traumatismo cranioencefálico em camundongos

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Neuroscience

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JoVE Journal Neuroscience

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Investigando alterações na microbiota de caecum após traumatismo cranioencefálico em camundongos

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04:29 min

September 19, 2019

DOI: 10.3791/59410-v

Liang Wen*¹, Wendong You*¹, Yadong Wang*¹, Yuanrun Zhu¹, Hao Wang¹, Xiaofeng Yang¹

¹Emergency and Trauma Center, The International Medical Center, The First Affiliated Hospital, School of Medicine,Zhejiang University

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Overview

This study presents a protocol for inducing diffuse traumatic brain injury (TBI) using a lateral fluid percussion device in mice. Following injury, the study investigates changes in gut microbiota by collecting caecum contents for analysis. The approach aims to elucidate the interactions between brain injury and gut microbiota composition.

Key Study Components

Area of Science

Neuroscience
Microbiome analysis
Traumatic brain injury research

Background

Understanding the relationships between the brain and gut microbiota is critical in TBI research.
Current knowledge of how TBI affects gut microbiota is limited.
The use of mice as a model organism allows for controlled experimental conditions.

Purpose of Study

To develop a reliable protocol for inducing TBI and analyzing subsequent changes in the gut microbiome.
To investigate potential correlations between neurological injury and gut microbiota diversity.
To provide insights into the impacts of TBI on overall health and microbiome interactions.

Methods Used

A lateral fluid percussion device was utilized to induce TBI in anesthetized mice.
Careful surgical procedures were undertaken, including skull penetration and cannula implantation.
Post-injury, caecum contents were collected for microbiome analysis and stored appropriately.
16S rDNA sequencing was employed to determine changes in microbiota diversity and composition.

Main Results

Analysis indicated reduced diversity in caecum microbiota within three days post-TBI.
Non-metric multidimensional scaling demonstrated significant shifts in microbiota composition following injury.
The study highlights the need for caution when handling tissues and maintaining sterile conditions.

Conclusions

This study establishes a methodology for exploring the gut-brain axis in the context of TBI.
Findings suggest that TBI may disrupt gut microbiota, which could have implications for recovery.
Enhancement of knowledge regarding gut-brain interactions could inform future therapeutic strategies for TBI.

Frequently Asked Questions

What are the advantages of using this TBI induction model?

The lateral fluid percussion method is relatively simple and efficient, facilitating controlled experiments to study TBI effects on the microbiome.

How is the brain injury induced in the study?

Anesthetized mice undergo cranial surgery, after which a lateral fluid percussion device administers a controlled injury.

What types of data are obtained from this study?

Data includes microbiome diversity and compositional changes analyzed through 16S rDNA sequencing.

How can this method be adapted for other research?

This protocol can be adapted to study other brain injuries or variations in microbiome analysis techniques.

What are key considerations when performing the surgical procedures?

Maintaining sterility and carefully manipulating tissues are crucial to avoid complications during the procedure.

É apresentado aqui um protocolo para induzir ferimento de cérebro traumático difuso usando um dispositivo de percussão fluido lateral seguido pela coleção do índice do cécum para a análise do microbioma do intestino.

A simultânea e as interações entre o cérebro e a microbiota intestinal permanecem desconhecidas. Use esta técnica, alterações na microbiota intestinal após lesão cerebral traumática em camundongos podem ser examinados. A principal vantagem dessa técnica é que ela é relativamente simples e eficiente.

Demonstrando o procedimento será Wendong Você, um estudante de pós-graduação do meu laboratório. Para indução traumática de lesão cerebral, confirme a falta de resposta ao beliscão do dedo do pé em um rato anestesiado, e aplique pomada nos olhos do animal. Após a barba, desinfete a pele exposta do couro cabeludo com 70% de etanol antes de incisar o couro cabeludo em um plano sagital.

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