Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / CRISPR/Cas9 Technology in Restoring Dystrophin…
JoVE Journal
Developmental Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Developmental Biology
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
Effect of Left Atrial Ligation on Avian Embryonic Hearts and HLHS Implications (Video) | JoVE
Journal (Developmental Biology)
Effect of Left Atrial Ligation on Avian Embryonic Hearts and HLHS Implications (Video) | JoVE
In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
Journal (Developmental Biology)
In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
 

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

Article DOI: 10.3791/59432-v • 07:44 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1, 1, 1
1Medical College of Georgia, Augusta University

Capítulos

Resumo
Automatic Translation

Here, we present a Cas9-based exon23 deletion protocol to restore dystrophin expression in iPSC from Dmdmdx mouse-derived skin fibroblasts and directly differentiate iPSCs into myogenic progenitor cells (MPC) using the Tet-on MyoD activation system.

Tags

CRISPR/Cas9 Technology Restoring Dystrophin Expression IPSC-derived Muscle Progenitors Duchenne Muscular Dystrophy Gene Editing Stem Cells Regeneration Autologous Therapy Genetic Correction Congenital Diseases Cell Reprogramming Gene Editing Technique
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter