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Hochauflösende Bildgebung von Kerndynamik in Live-Zellen unter Uniaxialer Tensile-Straße

Article DOI: 10.3791/59474-v • 09:20 min • June 2nd, 2019
June 2nd, 2019 •
1, 1,2, 1,2,3
1BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, CREATE, 2Department of Material Science and Engineering, Massachusetts Institute of Technology, 3Department of Biological Engineering, Massachusetts Institute of Technology

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Mit Hilfe eines zuvor entwickelten Geräts zur mechanischen Belastung von Anbauzellen beschreibt dieser Beitrag eine neu gestaltete Substratum-Geometrie und ein maßgeschneidertes Gerät zur hochauflösenden Einzeller-Abbildung von angespannten Zellen mit einem 100x Öl-Immersionsziel.

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Bioengineering Ausgabe 148 Zellstreckung Zugbelastung Mechanobiologie der Differenzierung Oligodendrozyten-Vorläuferzellen Kerndynamik Mechanotransduktion
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