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Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative
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Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative

Article DOI: 10.3791/59768-v • 05:53 min • June 13th, 2019
June 13th, 2019 •
1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

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The article describes a quick protocol for labeling blood vessels in a teleost fish by cardiac perfusion of DiI diluted in fixative, using medaka (Oryzias latipes) as a model and focusing on brain and pituitary tissue.

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Blood Vessels Teleost Brain Pituitary Cardiac Perfusion DiI-fixative Labeling Vascular Anatomy Neurons Endocrine Cells Fish Holder Polystyrene Perfusion System Plastic Cannula Glass Pipettes Dissection DiI-fixative Solution
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