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In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research

Article DOI: 10.3791/59810-v • 08:53 min • September 16th, 2019
September 16th, 2019 •
1, 2
1Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Centre Léon Bérard, 2Department of Radiology/Molecular Imaging Program at Stanford, School of Medicine, Stanford University

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The in vivo immunofluorescence localization (IVIL) method can be used to examine in vivo biodistribution of antibodies and antibody conjugates for oncological purposes in living organisms using a combination of in vivo tumor targeting and ex vivo immunostaining methods.

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In Vivo Immunofluorescence Localization IVIL Therapeutic Antibodies Diagnostic Antibodies Antibody-based Agents Cancer Research Biodistribution Immunofluorescent Staining Living Animal Workflow Protocol Cancer Model Rabbit Anti-mouse B7-H3 Antibody Rabbit IgG Isotype Control Antibody Desalting Column Dosages Microcentrifuge Tubes Tail Vein Inoculation Disinfect Heat Pad
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