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Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Article DOI: 10.3791/59916-v • 05:21 min • August 29th, 2019
August 29th, 2019 •
1, 1
1Graduate School of Horticulture, Chiba University

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Automatic Translation

We demonstrate a method to determine successful or failed fertilization on the basis of sperm nuclear morphology in Arabidopsis double fertilization using an epifluorescence microscope.

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Fertilization State Sperm Nuclear Morphology Arabidopsis Double Fertilization Evaluation Protocol Involvement Of Factors Morphology Of Sperm Nuclei Statistical Analysis Fertilization Phenotype Fertilization Regulators Functional Analysis Ovules Handling Arabidopsis Growth Conditions Emasculation Flower Buds DMP9-knockdown Line HTR10-mRFP Flower Pollination
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