Medindo interações entre sondas fluorescentes e lignina em seções de plantas por sFLIM Com base na autofluorescência nativa
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January 02, 2020
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Summary
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Este protocolo descreve uma configuração original que combina medições espectrais e fluorescência ao longo da vida para avaliar a transferência de energia de ressonância Förster (FRET) entre sondas fluorescentes à base de rodamina e polímero de lignina diretamente em seções de plantas espessas.
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Terryn, C., Habrant, A., Paës, G., Spriet, C. Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence. J. Vis. Exp. (155), e59925, doi:10.3791/59925 (2020).
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