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A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Article DOI: 10.3791/60150-v • 10:12 min • October 13th, 2019
October 13th, 2019 •
1,2,3, 1,2, 1,2
1Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University, 2Regenerative Medicine Center, University Medical Center Utrecht, Utrecht University, 3Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University

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This protocol describes an assay for the characterization of lipid droplet (LD) formation in human intestinal organoids upon stimulation with fatty acids. We discuss how this assay is used for quantification of LD formation, and how it can be used for high throughput screening for drugs that affect LD formation.

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Fluorescence-based Assay Lipid Droplet Formation Human Intestinal Organoids Quantification High-throughput Screening Drugs Modulate Lipid Droplet-specific Dye Biopsy-derived Intestinal Organoids Stable Model Accurate Model Physiologically-relevant Model Screen For Drug Candidates DGAT1-deficient Patients Lipid Metabolism Organoid Cultures Density Of Organoid Culture Consistent Organoid Density Visual Demonstration Culturing Organoids Handling Organoids Analysis Of Lipid Droplet Formation Preparing Organoids Culture Medium Aspiration Basement Membrane Matrix Disruption
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