Imagem tridimensional de células bacterianas para representações celulares precisas e localização precisa de proteínas
9,949 Views
•
06:33 min
•
October 29, 2019
DOI:
Summary
Automatically generated
Este protocolo explica como preparar e montar amostras bacterianas para imagens tridimensionais vivas e como reconstruir a forma tridimensional de E. coli a partir dessas imagens.
Vídeos Relacionados
10:26
Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
Vídeos Relacionados
13101 Views
05:57
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Vídeos Relacionados
7078 Views
08:47
Super-resolution Imaging of the Bacterial Division Machinery
Vídeos Relacionados
11805 Views
09:12
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Vídeos Relacionados
6941 Views
08:59
4D Imaging of Protein Aggregation in Live Cells
Vídeos Relacionados
17376 Views
05:46
3D Printing Bacteria to Study Motility and Growth in Complex 3D Porous Media
Vídeos Relacionados
2129 Views
12:44
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
Vídeos Relacionados
19965 Views
08:01
High-Resolution Three-Dimensional Whole-Organ Tomography of Microbial Infections
Vídeos Relacionados
878 Views
06:33
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Vídeos Relacionados
9.9K Views
07:30
Protocol for Producing Three-Dimensional Infrared Video of Freezing in Plants
Vídeos Relacionados
6.2K Views
Read Article
Cite this Article
Bratton, B. P., Barton, B., Morgenstein, R. M. Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization. J. Vis. Exp. (152), e60350, doi:10.3791/60350 (2019).
Copy