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Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Article DOI: 10.3791/60523-v • 07:07 min • June 27th, 2020
June 27th, 2020 •
*1, *1, 2,3, 1
1CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 2School of Life Science, University of Science and Technology of China, 3State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences
* These authors contributed equally

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This paper describes protocols of evaluating Tfh and GC B response in mouse model of influenza virus infection.

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T Follicular Helper Cells Germinal Center Response Influenza A Virus Infection Mice CD4 T Cells High Affinity Antibody Production Tfh-associated Studies Virus Titer Determination PR8 Virus Inoculation Biosafety Level 2 Conditions Virus Stock Thawing Virus Dilution Animal Anesthesia And Inoculation Procedure Monitoring And Weighing Of Mice Experimental Endpoint Procedures
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