Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Efficient Neural Differentiation using…
JoVE Journal
Developmental Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Developmental Biology
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
Journal (Developmental Biology)
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging
Journal (Developmental Biology)
Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging
 

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Article DOI: 10.3791/60571-v • 11:17 min • January 18th, 2020
January 18th, 2020 •
1, 2, 1
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Capítulos

Resumo
Automatic Translation

Presented here is a protocol for the generation of a single-cell culture of human embryonic stem cells and their subsequent differentiation into neural progenitor cells. The protocol is simple, robust, scalable, and suitable for drug screening and regenerative medicine applications.

Tags

Neural Differentiation Single-cell Culture Human Embryonic Stem Cells Culture Method Neural Progenitor Cells Neuron Astrocyte Oligodendrocyte Improved Culture System High-density Single Cell Type Culture Molecular Mechanisms Differentiated Lineages Basement Membrane Matrix DMEM/F-12 Feeder-free Cultures
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter