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JoVE Journal Developmental Biology
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Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Article DOI: 10.3791/60793-v • 09:13 min • February 14th, 2020
February 14th, 2020 •
1, 2, 1, 3,4,5, 1
1Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Department of Molecular Biotechnology, Turkish-German University, 3Institut für Mikrobiologie, Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research

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Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.

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Larval Zebrafish Infection Model Clostridioides Difficile Innate Immune System Macrophages Pathogen Recognition Phagocytosis Anaerobic Bacteria Oral Administration Spectrophotometer Cuvette OD600 Measurement Bacterial Suspension Fluorescent Dye Staining Low-melting Agarose Danieau's Medium
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