Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Keerat Kaur; Nishat Sultana; Yoav Hadas; Ajit Magadum; Mohammad Tofael Kabir Sharkar; Elena Chepurko; Lior Zangi

doi:10.3791/60832

Entrega de mRNA modificado em um modelo de mouse de infarto do miocárdio

JoVE Journal
Genetics

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JoVE Journal Genetics

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Entrega de mRNA modificado em um modelo de mouse de infarto do miocárdio

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06:03 min

June 11, 2020

DOI: 10.3791/60832-v

Keerat Kaur^1,2,3, Nishat Sultana^1,2,3, Yoav Hadas^1,2,3, Ajit Magadum^1,2,3, Mohammad Tofael Kabir Sharkar^1,2,3, Elena Chepurko^1,2,3, Lior Zangi^1,2,3

¹Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

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Overview

This protocol presents a novel method for transiently upregulating a gene of interest using modified RNA (modRNA) after myocardial infarction in mice. It provides a standardized approach for delivering genes to the heart, which can also be applied to other models of cardiac injury.

Key Study Components

Area of Science

Gene transfer
Cardiac research
Myocardial infarction

Background

Modified RNA is an innovative technique for gene delivery.
This method allows for transient expression of genes in the heart.
It has potential applications beyond myocardial infarction, including ischemia reperfusion and transverse aortic constriction models.
Currently, modified RNA is being utilized in various human clinical trials.

Purpose of Study

To provide a clear protocol for gene delivery using modRNA.
To enable accurate injection of modified RNA into the heart.
To standardize the approach for researchers in the field.

Methods Used

Delivery of modified RNA to the heart.
Injection techniques demonstrated visually.
Application of the method in various cardiac injury models.
Comparison with existing gene transfer methods.

Main Results

Successful transient upregulation of target genes in the heart.
Demonstration of effective injection sites for modRNA.
Potential for broader applications in cardiac research.
Establishment of a standardized protocol for future studies.

Conclusions

Modified RNA represents a promising tool for gene therapy in cardiac conditions.
This protocol can enhance the reproducibility of gene delivery studies.
Further research is needed to explore its full potential in clinical settings.

Frequently Asked Questions

What is modified RNA?

Modified RNA is a technique used for gene transfer, allowing for transient expression of genes in target tissues.

How is the protocol applied?

The protocol involves injecting modified RNA into specific sites in the heart after myocardial infarction.

Can this method be used for other conditions?

Yes, it can also be applied to ischemia reperfusion and transverse aortic constriction models.

What are the advantages of using modified RNA?

It allows for transient gene expression and has potential applications in various cardiac conditions.

Is there a standardized protocol for this method?

This study aims to establish a standardized protocol for gene transfer using modified RNA.

Are there any clinical trials using modified RNA?

Yes, modified RNA is currently being tested in several human clinical trials.

Este protocolo apresenta uma maneira simples e coerente de regulamentar transitoriamente um gene de interesse usando modRNA após infarto do miocárdio em camundongos.

RNA modificado é um novo método para transferência de genes para o coração, e outros órgãos. Este protocolo fornecerá uma abordagem precisa de entregar genes desejados transitoriamente ao coração, após infarto do miocárdio. Este método não se limita ao infarto do miocárdio, mas também pode ser aplicado à reperfusão de isquemia e modelos de constrição aórtica transversal.

O RNA modificado está sendo usado em vários ensaios clínicos em humanos. No entanto, não há um protocolo padronizado para gene transferido para o coração usando este método. A demonstração visual permitirá que os pesquisadores injetem corretamente o RNA modificado em locais apropriados do coração.

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