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Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
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Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

Article DOI: 10.3791/61154-v • 08:01 min • May 12th, 2020
May 12th, 2020 •
1, 1, 1, 2, 2, 1
1Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, 2Department of Physics, West Chester University

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Described here is a nanosphere lithography method for parallel fabrication of zero mode waveguides, which are arrays of nanoapertures in a metal-clad glass microscopy coverslip for single molecule imaging at nano- to micromolar concentrations of fluorophores. The method takes advantage of colloidal crystal self-assembly to create a waveguide template.

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Zero Mode Waveguides Single Molecule Microscopy Nanosphere Lithography Fabrication Low Cost Method Fluorescent Reagents Evaporative Deposition Colloidal Particles Borosilicate Glass Coverslips Coplin Glass Staining Jar Acetone Potassium Hydroxide Ethanol Forceps Petri Dish Centrifuge Polystyrene Beads
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